SFBA.social

3 posts3 participants0 posts today

Moritz NegwerHere's an interesting way to do photobleaching with cleared tissue: Add 0.1% H2O2 to the clearing medium and blast with LEDs for 24h at RT. Also includes very detailed instructions for building an LED photobleaching setup - the samples ate placed between two PCBs with LEDs in a ventilated box.Open-source Photobleacher for Fluorescent Imaging of Large Pigment-Rich Tissues Murakami et al., preprint at biorxiv 2025 <a href="https://www.biorxiv.org/content/10.1101/2025.02.24.639965v2.full.pdf" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://www.biorxiv.org/content/10.1101/2025.02.24.639965v2.full.pdf</a><a href="https://mstdn.science/tags/tissueclearing" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#tissueclearing</a> <a href="https://mstdn.science/tags/fluorescence" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#fluorescence</a> <a href="https://mstdn.science/tags/microscopy" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#microscopy</a> <a href="https://mstdn.science/tags/neuroscience" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#neuroscience</a>

Lukas VFN 🇪🇺New research finds <a href="https://scholar.social/tags/fluorescence" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#fluorescence</a> in feathers of Long-eared Owls <a href="https://phys.org/news/2025-04-fluorescence-feathers-eared-owls.html" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://phys.org/news/2025-04-fluorescence-feathers-eared-owls.html</a>Fluorescent pigment concentration correlated with age, sex, and size in <a href="https://scholar.social/tags/LongEaredOwl" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#LongEaredOwl</a> (Asio otus) plumage <a href="https://www.tandfonline.com/doi/full/10.1080/15594491.2024.2444031" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://www.tandfonline.com/doi/full/10.1080/15594491.2024.2444031</a> "female Long-eared <a href="https://scholar.social/tags/Owls" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Owls</a> have a much higher concentration of these pigments in their feathers, challenging a common misconception that colorful <a href="https://scholar.social/tags/plumage" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#plumage</a> is a male trait... We are only beginning to describe fluorescent pigments in <a href="https://scholar.social/tags/birds" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#birds</a> and other vertebrates"

Colin PurringtonI'm cleaning out a relative's house and one of the fun finds has been glassware that glows under UV light and thus might contain uranium. But fluorescence isn't especially bright so I have to find a Geiger counter to confirm. <a href="https://flipping.rocks/tags/uvlight" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#uvlight</a> <a href="https://flipping.rocks/tags/fluorescence" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#fluorescence</a> <a href="https://flipping.rocks/tags/uranium" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#uranium</a> <a href="https://flipping.rocks/tags/glass" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#glass</a> <a href="https://flipping.rocks/tags/glassware" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#glassware</a> <a href="https://flipping.rocks/tags/annagr%C3%BCn" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#annagrün</a> <a href="https://flipping.rocks/tags/annagelb" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#annagelb</a>

Eishi Noguchi<a href="https://masto.ai/tags/FluorescenceFriday" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#FluorescenceFriday</a>Front yard😊 Not really fluorescence, but like fluorescence 😉<a href="https://masto.ai/tags/Flower" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Flower</a> <a href="https://masto.ai/tags/Fluorescence" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Fluorescence</a> <a href="https://masto.ai/tags/Plant" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Plant</a> <a href="https://masto.ai/tags/Yard" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Yard</a> <a href="https://masto.ai/tags/Photography" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Photography</a>

Lukas VFN 🇪🇺Fluorescent <a href="https://scholar.social/tags/caves" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#caves</a> could explain how life can be supported in extraterrestrial environments <a href="https://phys.org/news/2025-03-fluorescent-caves-life-persists-extraterrestrial.html" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://phys.org/news/2025-03-fluorescent-caves-life-persists-extraterrestrial.html</a>"Typically, to understand the chemical makeup of a <a href="https://scholar.social/tags/cave" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#cave</a>, a sample is taken back to the lab. Sebree & team collect <a href="https://scholar.social/tags/fluorescence" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#fluorescence</a> spectra on their expeditions... they take the information but leave the cave intact. Anna Van Der Weide is building a public inventory of fluorescence fingerprints... Jacqueline Heggen is exploring the caves as a simulated environment for <a href="https://scholar.social/tags/astrobiology" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#astrobiology</a>"

Colin PurringtonI've recently learned that some glassware contains uranium dioxide and will glow under ultraviolet light. And that they're collectible. For giggles, I used my UV Beast to illuminate some bowls at a house I'm cleaning out. They are not at all yellow/green in normal light. Are they glowing enough? <a href="https://flipping.rocks/tags/uranium" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#uranium</a> <a href="https://flipping.rocks/tags/fluorescence" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#fluorescence</a> <a href="https://flipping.rocks/tags/uvlight" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#uvlight</a> <a href="https://flipping.rocks/tags/physics" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#physics</a> <a href="https://flipping.rocks/tags/chemistry" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#chemistry</a> <a href="https://flipping.rocks/tags/glass" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#glass</a>

Bose-Einstein-Kondensat<a href="https://mstdn.social/tags/DIYNAFLUOR" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#DIYNAFLUOR</a>: A portable <a href="https://mstdn.social/tags/OpenSource" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#OpenSource</a> <a href="https://mstdn.social/tags/DIY" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#DIY</a> plug-&-play nucleic acid <a href="https://mstdn.social/tags/fluorometer" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#fluorometer</a> for <a href="https://mstdn.social/tags/eDNA" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#eDNA</a> quantification:-cost: <$40 -solder-free assembly from <a href="https://mstdn.social/tags/3Dprinted" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#3Dprinted</a> partsPreprint: <a href="https://doi.org/10.1101/2024.12.16.626200" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://doi.org/10.1101/2024.12.16.626200</a> GitHub: <a href="https://github.com/traulab/DIYNAFLUOR/tree/main" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://github.com/traulab/DIYNAFLUOR/tree/main</a> <a href="https://mstdn.social/tags/DIYbio" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#DIYbio</a> <a href="https://mstdn.social/tags/lab" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#lab</a> <a href="https://mstdn.social/tags/instruments" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#instruments</a> <a href="https://mstdn.social/tags/FrugalScience" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#FrugalScience</a> <a href="https://mstdn.social/tags/fluorescence" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#fluorescence</a>

SPIENovel fiber-based <a class="hashtag" href="https://bsky.app/search?q=%23optofluidic" rel="nofollow noopener noreferrer" target="_blank">#optofluidic</a> chip designed to monitor absorbance and <a class="hashtag" href="https://bsky.app/search?q=%23fluorescence" rel="nofollow noopener noreferrer" target="_blank">#fluorescence</a> of phase-separating and aggregating proteins. 💡 Read more about the design in <a class="hashtag" href="https://bsky.app/search?q=%23SPIE_JOM" rel="nofollow noopener noreferrer" target="_blank">#SPIE_JOM</a> <a class="hashtag" href="https://bsky.app/search?q=%23OpenAccess" rel="nofollow noopener noreferrer" target="_blank">#OpenAccess</a> - <a href="https://www.spiedigitallibrary.org/journals/journal-of-optical-microsystems/volume-5/issue-01/014004/Optofluidic-lab-on-chip-for-absorbance-and-fluorescence-analysis-in/10.1117/1.JOM.5.1.014004.full?utm_campaign=bluesky&utm_medium=social_networking&utm_source=customer_centric_multi_product" rel="nofollow noopener noreferrer" target="_blank">www.spiedigitallibra...</a>

Bose-Einstein-Kondensat<a href="https://mstdn.social/tags/OpenSsource" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#OpenSsource</a> implementation of <a href="https://mstdn.social/tags/polarisation" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#polarisation</a>-resolved single-shot differential <a href="https://mstdn.social/tags/phase" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#phase</a> contrast <a href="https://mstdn.social/tags/microscopy" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#microscopy</a> (<a href="https://mstdn.social/tags/pDPC" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#pDPC</a>) on a modular <a href="https://mstdn.social/tags/openFrame" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#openFrame</a>-based <a href="https://mstdn.social/tags/microscope" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#microscope</a>:-cost: ∼£2.5 k<a href="https://doi.org/10.1016/j.ohx.2024.e00622" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://doi.org/10.1016/j.ohx.2024.e00622</a> <a href="https://mstdn.social/tags/DIYbio" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#DIYbio</a> <a href="https://mstdn.social/tags/lab" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#lab</a> <a href="https://mstdn.social/tags/instruments" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#instruments</a> <a href="https://mstdn.social/tags/QPI" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#QPI</a> <a href="https://mstdn.social/tags/imaging" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#imaging</a> <a href="https://mstdn.social/tags/fluorescence" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#fluorescence</a> <a href="https://mstdn.social/tags/infrared" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#infrared</a>

Steven Saus [he/him]From 23 Feb: Flashy exotic birds can actually glow in the dark - The birds, known for their showy displays, look even more... <a href="https://arstechnica.com/science/2025/02/flashy-exotic-birds-can-actually-glow-in-the-dark/" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://arstechnica.com/science/2025/02/flashy-exotic-birds-can-actually-glow-in-the-dark/</a> <a href="https://faithcollapsing.com/tags/biology" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#biology</a> <a href="https://faithcollapsing.com/tags/bioluminescence" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#bioluminescence</a> <a href="https://faithcollapsing.com/tags/birds" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#birds</a> <a href="https://faithcollapsing.com/tags/feathers" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#feathers</a> <a href="https://faithcollapsing.com/tags/fluorescence" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#fluorescence</a> <a href="https://faithcollapsing.com/tags/science" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#science</a>

Lukas VFN 🇪🇺Study Finds That <a href="https://scholar.social/tags/BirdsOfParadise" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#BirdsOfParadise</a> Are Biofluorescent <a href="https://www.amnh.org/about/press-center/birds-of-paradise-biofluorescent" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://www.amnh.org/about/press-center/birds-of-paradise-biofluorescent</a> paper: <a href="https://royalsocietypublishing.org/doi/10.1098/rsos.241905" rel="nofollow noopener noreferrer" translate="no" target="_blank">https://royalsocietypublishing.org/doi/10.1098/rsos.241905</a>"an organism absorbs light, transforms it, and emits it as a different color... This phenomenon is especially prominent in males, focused on their bright plumage and skin in areas that are highlighted during displays: the inner mouth and bill, feet, and feathers on the head, neck, and belly... pigments in the <a href="https://scholar.social/tags/birds" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#birds</a>’ eyes align with the <a href="https://scholar.social/tags/fluorescence" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#fluorescence</a> peaks"<a href="https://scholar.social/tags/Biofluorescence" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Biofluorescence</a> <a href="https://scholar.social/tags/Animals" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#Animals</a>

ErikA wider view of the <a href="https://fosstodon.org/tags/fluorescence" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#fluorescence</a> from these flowers under 365nm <a href="https://fosstodon.org/tags/UV" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#UV</a> light, showing a more varied range of colors than just blue.<a href="https://fosstodon.org/tags/macrophotography" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#macrophotography</a> <a href="https://fosstodon.org/tags/flowers" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#flowers</a> <a href="https://fosstodon.org/tags/uvphotography" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#uvphotography</a> <a href="https://fosstodon.org/tags/photography" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#photography</a>

ErikWhile working on more multispectral images, I noticed the ethereal blue <a href="https://fosstodon.org/tags/fluorescence" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#fluorescence</a> of these flowers under ultraviolet light. This was done with 10 seconds of light painting with a 365nm UV flashlight, captured using an unmodified mirrorless camera. <a href="https://fosstodon.org/tags/uvphotography" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#uvphotography</a> <a href="https://fosstodon.org/tags/photography" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#photography</a> <a href="https://fosstodon.org/tags/macrophotography" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#macrophotography</a>

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#fluorescence